Reporter

Part:BBa_K1135004:Experience

Designed by: Ranvir Chaudhri   Group: iGEM13_UCL_PG   (2013-09-20)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1135004

Tiny scale (96 wells plate) living cell kinetic study of glucose level. (Works even better in human cell culture!)

User Reviews

UNIQ2c09b419b748201d-partinfo-00000000-QINU UCL_PG 2013:
Preceding pCSTA biobrick [BBa_K118011]
Preceding pCSTA.RBS.eGFP.TT [BBa_K200018]
Preceding pCSTA.RBS.mRFP.TT [BBa_K572001]

pCSTA.RBS.mKeima.TT is compared with the preceding biobricks. We actually reconstructed pCSTA.RBS.eGFP.TT and pCSTA.RBS.mRFP.TT from 2013 distribution to test the modularity of the generator bricks. They are all successfully clone in few days.
During the comparison, we used a low volume of living E.coli for fluorescent detection. They are not lysed. The purpose is to demonstrate suitability of each fluorescent protein in tiny scale kinetic study. We improved the pCSTA based glucose sensor's sensitivity and demonstrated that mKeima is a better FP for indicating glucose level in culture. Please refer to our project page for more!



UNIQ2c09b419b748201d-partinfo-00000002-QINU